Kiwi+and+DNA

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Aim: To extract DNA from kiwi fruit

Diagram: Mixture of salt(3g), washing up liquid(10ml) and 100ml of water, Kiwi, 250ml beaker, Water bath (60 degree Celsius)

__KIWI MUSH__ Method Gather all the equipment shown in the diagram. Start chopping up the half-a-kiwi until it was mush. Another person go get 10ml of washing up liquid and pour it into the 250ml beaker with salt and 100ml water. - The salt and washing up liquid is put in the beaker to break down the cell membrane. Stir it to dissolve the salt - Be careful not to make frothy bubbles on the water. Then, mixed it with the kiwi mush Put the beaker in the water bath/boiler set in 60degree Celsius for 15 minutes. After 15 minutes, take it out and put it in an ice bath for 2 minutes to cool it down. Gather a funnel, round sheet of paper(make in a cone shape) and a small beaker. Filter the liquid and wait until the DNA extracted(approximately 50ml) Put it in a fridge over night. The next morning, pour 25ml of cold ethanol into the extract(kiwi juice) and took some out using a needle. Put it on a tile. Pour a drop of universal indicator to observe the DNA clearly.

Results When applying the universal indicator to the DNA, it turns to yellowish-orange colour.

Conclusion Kiwi has DNA. The salt, detergent and temperature was to break the cell membrane and the DNA would be destroyed if it was heated for too long.